Coding

Part:BBa_K4231001

Designed by: yang qingyan   Group: iGEM22_USTC   (2022-09-30)


ERG13 of Yarrowia lipolytica

The coding sequence of ERG13, the second enzyme of MVA pathway in Yarrowia lipolytica. ERG13 condenses acetyl-CoA with acetoacetyl-CoA to form hydroxymethylglutaryl-CoA (HMG-CoA).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1042
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 38
    Illegal XhoI site found at 34
    Illegal XhoI site found at 76
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1042
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1042
    Illegal NgoMIV site found at 484
    Illegal AgeI site found at 970
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None